{"id":1957,"date":"2025-07-08T06:16:07","date_gmt":"2025-07-08T06:16:07","guid":{"rendered":"https:\/\/bstextract.com\/?p=1957"},"modified":"2025-07-08T06:16:37","modified_gmt":"2025-07-08T06:16:37","slug":"%e3%82%a4%e3%83%b3%e3%83%99%e3%83%ab%e3%82%bf%e3%83%bc%e3%82%bc%e6%a9%9f%e8%83%bd","status":"publish","type":"post","link":"https:\/\/bstextract.com\/ja\/%e3%82%a4%e3%83%b3%e3%83%99%e3%83%ab%e3%82%bf%e3%83%bc%e3%82%bc%e6%a9%9f%e8%83%bd\/","title":{"rendered":"\u69cb\u9020\u7684\u6d1e\u5bdf\u3068\u9175\u7d20\u5de5\u5b66\uff1a\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u306e\u6a5f\u80fd\u3068\u6700\u9069\u5316"},"content":{"rendered":"<h2><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter wp-image-1958\" src=\"https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/images.webp\" alt=\"\" width=\"1200\" height=\"408\" title=\"\" srcset=\"https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/images.webp 385w, https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/images-300x102.webp 300w, https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/images-18x6.webp 18w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/><\/h2>\n<h2><strong>\u306f\u3058\u3081\u306b\u9175\u7d20\u30a4\u30ce\u30d9\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3051\u308b\u69cb\u9020\u751f\u7269\u5b66\u306e\u529b<\/strong><\/h2>\n<p>\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u306e\u3088\u3046\u306a\u9175\u7d20\u306e\u69cb\u9020\u3092\u89e3\u304d\u660e\u304b\u3059\u3053\u3068\u306f\u3001\u5b66\u8853\u7684\u306a\u610f\u5473\u3092\u8d85\u3048\u3066\u3044\u308b\u3002 <strong>\u3088\u308a\u30b9\u30de\u30fc\u30c8\u3067\u6301\u7d9a\u53ef\u80fd\u306a\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/strong>.\u3092\u901a\u3057\u3066 <strong>\u90e8\u4f4d\u7279\u7570\u7684\u7a81\u7136\u5909\u7570\u8a98\u767a<\/strong> \u305d\u3057\u3066 <strong>\u30bf\u30f3\u30d1\u30af\u8cea\u5de5\u5b66<\/strong>\u79c1\u305f\u3061\u306f\u3001\u9175\u7d20\u306e\u5b89\u5b9a\u6027\u3001\u52b9\u7387\u3001\u5de5\u696d\u7684\u9069\u5fdc\u6027\u3092\u9ad8\u3081\u3066\u3044\u308b\u3002\u3053\u308c\u3089\u306e\u65b9\u6cd5\u306b\u3088\u3063\u3066\u3001\u5929\u7136\u9175\u7d20\u304c\u3069\u306e\u3088\u3046\u306b\u5f37\u529b\u306a\u751f\u4f53\u89e6\u5a92\u306b\u5909\u8eab\u3059\u308b\u306e\u304b\u3092\u63a2\u3063\u3066\u307f\u3088\u3046\u3002<\/p>\n<h2><strong>\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u306e\u69cb\u9020\u3068\u6a5f\u80fd\u3092\u7406\u89e3\u3059\u308b<\/strong><\/h2>\n<p>\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\uff08\u03b2-\u30d5\u30eb\u30af\u30c8\u30d5\u30e9\u30ce\u30b7\u30c0\u30fc\u30bc\uff09\u306f\u3001\u30b9\u30af\u30ed\u30fc\u30b9\u3092\u30b0\u30eb\u30b3\u30fc\u30b9\u3068\u30d5\u30eb\u30af\u30c8\u30fc\u30b9\u306b\u52a0\u6c34\u5206\u89e3\u3059\u308b\u3002 <strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27871374\/\" rel=\"nofollow noopener\" target=\"_blank\">\u6d3b\u6027\u90e8\u4f4d\u30ab\u30eb\u30dc\u30f3\u9178\u6b8b\u57fa<\/a><\/strong>\u57fa\u8cea\u8a8d\u8b58\u3068\u89e6\u5a92\u53cd\u5fdc\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3002\u305d\u306e\u4e2d\u3067 <em>\u30b5\u30c3\u30ab\u30ed\u30de\u30a4\u30bb\u30b9\u30fb\u30bb\u30ec\u30d3\u30b7\u30a8<\/em>\u3092\u5f62\u6210\u3059\u308b\u3002 <strong><a href=\"https:\/\/en.wikipedia.org\/wiki\/Invertase\" rel=\"nofollow noopener\" target=\"_blank\">8\u91cf\u4f534\u6b21\u69cb\u9020<\/a><\/strong>\u5b89\u5b9a\u6027\u3068\u57fa\u8cea\u7d50\u5408\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u300c\u30aa\u30fc\u30d7\u30f3\u300d\u4e8c\u91cf\u4f53\u3068\u300c\u30af\u30ed\u30fc\u30ba\u300d\u4e8c\u91cf\u4f53\u306b\u7d44\u7e54\u5316\u3055\u308c\u308b\u3002<\/p>\n<h2><strong>\u5408\u7406\u7684\u306a\u90e8\u4f4d\u7279\u7570\u7684\u7a81\u7136\u5909\u7570\u8a98\u767a\uff1a\u6a5f\u80fd\u3092\u6700\u9069\u5316\u3059\u308b\u305f\u3081\u306e\u7cbe\u5bc6\u306a\u5fae\u8abf\u6574<\/strong><\/h2>\n<p>\u7814\u7a76\u8005\u305f\u3061\u306f\u3001\u3088\u304f\u77e5\u3089\u308c\u305fSUC2\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3092\u6a19\u7684\u3068\u3057\u3001\u8010\u71b1\u6027\u3068\u89e6\u5a92\u6027\u80fd\u3092\u9ad8\u3081\u305f (<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27871374\/\" rel=\"nofollow noopener\" target=\"_blank\">\u30d1\u30d6\u30e1\u30c9\u7814\u7a76<\/a>):<\/p>\n<ul>\n<li><strong>P152V\uff08Mut1\uff09\u306e\u3088\u3046\u306a\u5909\u7570\u4f53<\/strong> \u89e6\u5a92\u52b9\u7387\u304c\u5411\u4e0a\u3057\u3001kcat\/Km\u304c54%\u5897\u52a0\u3057\u305f\u3002<\/li>\n<li>\u8907\u5408\u7f6e\u63db\uff08\u4f8b\u3048\u3070Mut4\u306eS305V\/N463V\uff09\u306f\u3001\u301c16%\u8010\u71b1\u6027\u3092\u5897\u52a0\u3055\u305b\u305f\u3002<\/li>\n<li>\u6226\u7565\u7684\u306a\u914d\u7f6e <strong>\u758e\u6c34\u6027\u6b8b\u57fa<\/strong> \u6d3b\u6027\u90e8\u4f4d\u306e\u30eb\u30fc\u30d7\u304c\u91cd\u8981\u3067\u3042\u308b\u3053\u3068\u304c\u5224\u660e\u3057\u305f\u3002<\/li>\n<\/ul>\n<p><img decoding=\"async\" class=\"aligncenter size-large wp-image-1952\" src=\"https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/vitamin-d-3091385_1280-1024x667.webp\" alt=\"\" width=\"800\" height=\"521\" title=\"\" srcset=\"https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/vitamin-d-3091385_1280-1024x667.webp 1024w, https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/vitamin-d-3091385_1280-300x195.webp 300w, https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/vitamin-d-3091385_1280-768x500.webp 768w, https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/vitamin-d-3091385_1280-18x12.webp 18w, https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/vitamin-d-3091385_1280-600x391.webp 600w, https:\/\/bstextract.com\/wp-content\/uploads\/2025\/07\/vitamin-d-3091385_1280.webp 1280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/p>\n<h2><strong>\u7d44\u63db\u3048\u767a\u73fe\u30b7\u30b9\u30c6\u30e0\u30b3\u30c9\u30f3\u6700\u9069\u5316\u3068\u5bbf\u4e3b\u9078\u629e<\/strong><\/h2>\n<p>\u52b9\u7387\u7684\u306a\u7d44\u63db\u3048\u767a\u73fe\u304c\u9375\u3068\u306a\u308b\uff1a<\/p>\n<ul>\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27871374\/\" rel=\"nofollow noopener\" target=\"_blank\">\u30d4\u30ad\u30a2\u30fb\u30d1\u30b9\u30c8\u30ea\u30b9<\/a><\/strong> \u306f\u826f\u597d\u306a\u6d3b\u6027\u3068\u8010\u71b1\u6027\u3092\u4fdd\u6301\u3057\u3001\u301c60\u2103\u306e\u81f3\u9069\u6d3b\u6027\u3092\u7dad\u6301\u3059\u308b\u3002<\/li>\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26047917\/\" rel=\"nofollow noopener\" target=\"_blank\">\u5927\u8178\u83cc<\/a><\/strong> \u306e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3067\u793a\u3055\u308c\u305f\u3088\u3046\u306b\u3001\u30b3\u30c9\u30f3\u30b3\u30f3\u30c6\u30ad\u30b9\u30c8\u306e\u6700\u9069\u5316\u306f\u3001\u30b7\u30b9\u30c6\u30e0\u306b\u3068\u3063\u3066\u6709\u76ca\u3067\u3042\u308b\u3002 <em>\u30b5\u30fc\u30e2\u30c8\u30ac\u30fb\u30de\u30ea\u30c1\u30de<\/em>.<\/li>\n<\/ul>\n<h2><strong>\u8010\u71b1\u6027\u306e\u5411\u4e0a\u5206\u5b50\u30fb\u5316\u5b66\u5de5\u5b66<\/strong><\/h2>\n<p>\u7a81\u7136\u5909\u7570\u4ee5\u5916\u306b\u3082\u3001\u9175\u7d20\u3092\u5b89\u5b9a\u5316\u3055\u305b\u308b\u65b9\u6cd5\u304c\u3042\u308b\uff1a<\/p>\n<ul>\n<li><strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19918887\/\" rel=\"nofollow noopener\" target=\"_blank\">\u5316\u5b66\u67b6\u6a4b<\/a><\/strong> \u30b8\u30a4\u30bd\u30b7\u30a2\u30cd\u30fc\u30c8\u3068\u306e\u4f75\u7528\u306b\u3088\u308a\u300160\u2103\u3067\u306e\u71b1\u5909\u6027\u7387\u304c72%\u6e1b\u5c11\u3057\u305f\u3002<\/li>\n<li><strong><a href=\"https:\/\/en.wikipedia.org\/wiki\/Directed_evolution\" rel=\"nofollow noopener\" target=\"_blank\">\u6307\u793a\u9032\u5316<\/a><\/strong>\u6a5f\u68b0\u5b66\u7fd2\u306b\u3088\u3063\u3066\u5f37\u5316\u3055\u308c\u308b\u53ef\u80fd\u6027\u306e\u3042\u308b\u3001\u5408\u7406\u7684\u306a\u8a2d\u8a08\u3092\u88dc\u5b8c\u3059\u308b\u3082\u306e\u3067\u3042\u308b\u3002<\/li>\n<\/ul>\n<h2><strong>\u7523\u696d\u30ec\u30b8\u30ea\u30a8\u30f3\u30b9\u306e\u305f\u3081\u306e\u30a8\u30f3\u30b8\u30cb\u30a2\u30ea\u30f3\u30b0<\/strong><\/h2>\n<p>\u6210\u529f\u4f8b\u306f\u4ee5\u4e0b\u306e\u901a\u308a\uff1a<\/p>\n<ul>\n<li>\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3092\u4ecb\u3057\u305f <strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36201138\/\" rel=\"nofollow noopener\" target=\"_blank\">\u30a8\u30b9\u30c7\u30a3\u30fc\u30a8\u30e0<\/a><\/strong> \u3067\u751f\u5730\u767a\u9175\u6d3b\u6027\u3092\u5411\u4e0a\u3055\u305b\u3001\uff5e52%\u3092\u5897\u52a0\u3055\u305b\u305f\u3002<\/li>\n<li>\u8010\u71b1\u6027\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc <strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26047917\/\" rel=\"nofollow noopener\" target=\"_blank\">\u30b3\u30c9\u30f3\u6700\u9069\u5316\u907a\u4f1d\u5b50<\/a><\/strong> \u5927\u8178\u83cc*\u3067\u5805\u7262\u306b\u4f7f\u7528\u3059\u308b\u3002<\/li>\n<\/ul>\n<h2><strong>\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u56fa\u5b9a\u5316\uff1a\u518d\u5229\u7528\u6027\u3068\u6027\u80fd\u306e\u5411\u4e0a<\/strong><\/h2>\n<p>\u7d44\u63db\u3048\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3092\u56fa\u5b9a\u5316\u3059\u308b\u3002 <em>\u30b8\u30e0\u30e2\u30ca\u30b9\u30e2\u30d3\u30ea\u30b9<\/em>)\u3092\u30ca\u30a4\u30ed\u30f3-6\u30d3\u30fc\u30ba\u306b\u5857\u5e03\u3057\u305f\uff1a<\/p>\n<ul>\n<li>\u8010\u71b1\u6027\u304c\u5411\u4e0a-50%\u306f30\u2103\u306870\u2103\u3067\u3088\u308a\u5b89\u5b9a(<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/18712547\/\" rel=\"nofollow noopener\" target=\"_blank\">\u30d1\u30d6\u30b3\u30e1\u51fa\u5178<\/a>).<\/li>\n<li>\u6d3b\u6027\u3092\u7dad\u6301\u3057\u305f\u307e\u307epH\uff5e5.5\u306b\u8010\u3048\u3001\u9023\u7d9a\u30b7\u30b9\u30c6\u30e0\u306b\u6700\u9069\u3002<\/li>\n<\/ul>\n<h2><strong>\u7523\u696d\u30a4\u30f3\u30c6\u30b0\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u5b9f\u8df5\u7684\u30d9\u30b9\u30c8\u30d7\u30e9\u30af\u30c6\u30a3\u30b9<\/strong><\/h2>\n<ol>\n<li>\u6d3b\u6027\u90e8\u4f4d\u306e\u91cd\u8981\u306a\u6b8b\u57fa\u306b\u3064\u3044\u3066\u9175\u7d20\u69cb\u9020\u3092\u30de\u30c3\u30d7\u3059\u308b\u3002<\/li>\n<li>SDM\u3092\u4f7f\u7528\u3057\u3066\u89e6\u5a92\u90e8\u4f4d\u3092\u6700\u9069\u5316\u3059\u308b\u3002<\/li>\n<li>\u7406\u60f3\u7684\u306a\u767a\u73fe\u5bbf\u4e3b\u3092\u9078\u629e\u3059\u308b\uff1a*P.\u30d1\u30b9\u30c8\u30ea\u30b9*\u307e\u305f\u306f*\u5927\u8178\u83cc*\u3002<\/li>\n<li>\u3088\u308a\u826f\u3044\u767a\u73fe\u306e\u305f\u3081\u306b\u907a\u4f1d\u5b50\u3092\u30b3\u30c9\u30f3\u6700\u9069\u5316\u3059\u308b\u3002<\/li>\n<li>\u5316\u5b66\u7684\/\u56fa\u5b9a\u5316\u6280\u8853\u306b\u3088\u308a\u9175\u7d20\u3092\u5b89\u5b9a\u5316\u3055\u305b\u308b\u3002<\/li>\n<li>\u8907\u96d1\u306a\u6700\u9069\u5316\u306e\u305f\u3081\u306eML\u30ac\u30a4\u30c9\u4ed8\u304d\u9032\u5316\u3092\u63a2\u6c42\u3059\u308b\u3002<\/li>\n<\/ol>\n<h2><strong>\u30d1\u30fc\u30c8\u30ca\u30fc <a href=\"https:\/\/bstextract.com\/ja\" rel=\"follow\">BSTBIO<\/a> \u9175\u7d20\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u306e\u30ab\u30b9\u30bf\u30de\u30a4\u30ba<\/strong><\/h2>\n<p>\u3067 <a href=\"https:\/\/bstextract.com\/ja\" rel=\"follow\">BSTBIO<\/a>\u79c1\u305f\u3061\u306e <a href=\"https:\/\/bstextract.com\/ja\/%e8%a3%bd%e5%93%81\/%e3%82%a4%e3%83%b3%e3%83%99%e3%83%ab%e3%82%bf%e3%83%bc%e3%82%bc%e9%85%b5%e7%b4%a0\/\" rel=\"follow\">\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u9175\u7d20<\/a> \u3092\u542b\u3080\uff1a<\/p>\n<ul>\n<li><strong>\u9ad8\u5ea6\u306a\u30a8\u30f3\u30b8\u30cb\u30a2\u30ea\u30f3\u30b0\u30fb\u30b5\u30dd\u30fc\u30c8<\/strong> \u304a\u5ba2\u69d8\u306e\u7528\u9014\u306b\u5408\u308f\u305b\u305f<\/li>\n<li>\u30aa\u30d7\u30b7\u30e7\u30f3 <strong>\u30df\u30e5\u30fc\u30bf\u30f3\u30c8\u30fb\u30c7\u30b6\u30a4\u30f3<\/strong>\u30b3\u30c9\u30f3\u306e\u6700\u9069\u5316\u3001\u307e\u305f\u306f\u56fa\u5b9a\u5316\u3002<\/li>\n<li><strong>\u5171\u540c\u7814\u7a76\u958b\u767a<\/strong> \u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9\u3092\u5411\u4e0a\u3055\u305b\u308b\u9175\u7d20\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u63d0\u4f9b\u3059\u308b\u3002<\/li>\n<\/ul>\n<h2><strong>\u9175\u7d20\u3092\u5909\u3048\u308b\u6e96\u5099\u306f\u3067\u304d\u3066\u3044\u308b\u304b\uff1f<\/strong><\/h2>\n<p><strong>\u9023\u7d61\u5148 <a href=\"https:\/\/bstextract.com\/ja\" rel=\"follow\">BSTBIO<\/a><\/strong> \u306b\uff1a<\/p>\n<ul>\n<li>\u7cbe\u88fd\u307e\u305f\u306f\u88fd\u5264\u5316\u53ef\u80fd\u306a\u30b5\u30f3\u30d7\u30eb\u3092\u3054\u8acb\u6c42\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u5909\u7570\u5c0e\u5165\u307e\u305f\u306f\u56fa\u5b9a\u5316\u306b\u3088\u308a\u30ab\u30b9\u30bf\u30e0\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3092\u8a2d\u8a08\u3059\u308b\u3002<\/li>\n<li>\u98df\u54c1\u3001\u88fd\u85ac\u3001\u5de5\u696d\u7528\u9014\u3067\u306e\u8a66\u9a13\u7684\u5171\u540c\u7814\u7a76\u3092\u691c\u8a0e\u3059\u308b\u3002<\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p>\u306f\u3058\u3081\u306b\u9175\u7d20\u30a4\u30ce\u30d9\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3051\u308b\u69cb\u9020\u751f\u7269\u5b66\u306e\u529b 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